Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, C6 cells, human brain tissue |
| Positive IF/ICC detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
Product Information
14071-1-AP targets Nicastrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nicastrin fusion protein Ag5137 相同性解析による交差性が予測される生物種 |
| Full Name | nicastrin |
| Calculated molecular weight | 709 aa, 78.4 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC047621 |
| Gene Symbol | Nicastrin |
| Gene ID (NCBI) | 23385 |
| RRID | AB_2149583 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92542 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nicastrin antibody 14071-1-AP | Download protocol |
| IF protocol for Nicastrin antibody 14071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gut Microbes Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis | ||
J Neuroinflammation Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice. | ||
Front Oncol Stimulation of Let-7 Maturation by Metformin Improved the Response to Tyrosine Kinase Inhibitor Therapy in an m6A Dependent Manner. | ||
Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. | ||
Cancer Cell Int Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation.
| ||
Neurosci Lett Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus. |