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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCX1 Polyclonal antibody (28447-1-AP)

    WB analysis using 28447-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NCX1 Polyclonal antibody (28447-1-AP)

    WB analysis using 28447-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NCX1 Polyclonal antibody (28447-1-AP)

    IHC staining of human kidney using 28447-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NCX1 Polyclonal antibody (28447-1-AP)

    IHC staining of human kidney using 28447-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NCX1 Polyclonal antibody

NCX1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28447-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Solute carrier family 8 member 1, SLC8A1, Na(+)/Ca(2+)-exchange protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue, rat kidney tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

28447-1-AP targets NCX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCX1 fusion protein Ag28952 相同性解析による交差性が予測される生物種
Full Name solute carrier family 8 (sodium/calcium exchanger), member 1
Calculated molecular weight 109 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_021097
Gene Symbol NCX1
Gene ID (NCBI) 6546
RRIDAB_2918167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCX1 antibody 28447-1-APDownload protocol
IHC protocol for NCX1 antibody 28447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain

Authors - Yun Li
View Article
mouseWB

Acta Pharmacol Sin

Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling

Authors - Hao Wu
View Article