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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDE1 Recombinant antibody (84799-4-RR)

    WB analysis using 84799-4-RR (same clone as 84799-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84799-4-RR (NDE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01589-1

    Sandwich ELISA standard curve of MP01589-1

    Sandwich ELISA standard curve of MP01589-1, Human NDE1 Recombinant Matched Antibody Pair - PBS only. 84799-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0336. 84799-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01589-1

    Sample test of MP01589-1

    The mean NDE1 concentration was determined to be 38.38 ng/mL in HepG2 cell extract based on a 2.50 mg/mL extract load, 43.20 ng/mL in K-562 cell extract based on a 1.00 mg/mL extract load and 22.42 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NDE1 Recombinant antibody (84799-4-RR)

    IHC staining of mouse liver using 84799-4-RR (same clone as 84799-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NDE1 Recombinant antibody (84799-4-RR)

    IHC staining of mouse liver using 84799-4-RR (same clone as 84799-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDE1 Recombinant antibody (84799-4-RR)

    IF Staining of HepG2 using 84799-4-RR (same clone as 84799-4-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NDE1 antibody (84799-4-RR, Clone: 242340H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84799-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01589-1

    Cytometric bead array standard curve of MP01589-1

    Cytometric bead array standard curve of MP01589-1, NDE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84799-2-PBS. Detection antibody: 84799-4-PBS. Standard: Ag0336. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84799-4-RR

    Affinity and Kinetic Characterization of 84799-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84799-4-RR against Human NDE1 were performed. The affinity constant is 0.342 nM.

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NDE1 Recombinant antibody, PBS Only (Detector)

NDE1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84799-4-PBS
Clone No.242340H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242340H4, HOM TES 87, NudE, NUDE1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84799-4-PBS targets NDE1 as part of a matched antibody pair:

MP01589-1: 84799-2-PBS capture and 84799-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDE1 fusion protein Ag0336 相同性解析による交差性が予測される生物種
Full Name nudE nuclear distribution gene E homolog 1 (A. nidulans)
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC001421
Gene Symbol NDE1
Gene ID (NCBI) 54820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXR1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center.