• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDNL2 Polyclonal antibody

NDNL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27488-1-AP

Synonyms

HCA4, MAGE G1 antigen, MAGEG1, MAGEL3, Melanoma associated antigen G1, NDNL2, necdin like 2, Necdin like protein 2, NSE3, NSMCE3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using NDNL2 Polyclonal antibody (27488-1-AP)

    WB analysis of HepG2 using 27488-1-AP

    HepG2, L02 and HeLa cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NDNL2 Polyclonal antibody (27488-1-AP)

    WB analysis of L02 using 27488-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDNL2 Polyclonal antibody (27488-1-AP)

    WB analysis of HeLa using 27488-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using NDNL2 Polyclonal antibody (27488-1-AP)

    WB analysis of human placenta using 27488-1-AP

    HepG2 cells and human placenta tissue were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDNL2 Polyclonal antibody (27488-1-AP)

    IHC staining of human liver cancer using 27488-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27488-1-AP (NDNL2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDNL2 Polyclonal antibody (27488-1-AP)

    IHC staining of human liver cancer using 27488-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27488-1-AP (NDNL2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDNL2 Polyclonal antibody (27488-1-AP)

    IF Staining of HepG2 using 27488-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27488-1-AP (NDNL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27488-1-AP targets NDNL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDNL2 fusion protein Ag26663 相同性解析による交差性が予測される生物種
Full Name necdin-like 2
Calculated molecular weight 34 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC053999
Gene symbol NDNL2
Gene ID (NCBI) 56160
RRIDAB_2880885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDNL2 (also known as NSMCE3 or MAGEG1) is a part of the SMC5-6 protein complex that is essential for DNA damage response and chromosome segregation (PMID: 18086888; 27427983). The gene of human NDNL2 maps to chromosome 15q13.1. Northern blot analysis detected expression of a 1.9-kb transcript in all human tissues tested, with highest expression in testis (PMID: 11782285). Missense mutations in NSMCE3 have been associated with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood (PMID: 27427983).

Protocols

Product Specific Protocols
WB protocol for NDNL2 antibody 27488-1-APDownload protocol
IHC protocol for NDNL2 antibody 27488-1-APDownload protocol
IF protocol for NDNL2 antibody 27488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu
View Article