NDOR1/NR1 Polyclonal antibody

Name: NDOR1/NR1 Polyclonal antibody
Brand: Proteintech
SKU: 11404-1-AP

NDOR1/NR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11404-1-AP

Synonyms

NDOR1, Novel reductase 1, NADPH-dependent FMN and FAD-containing oxidoreductase, NADPH-dependent diflavin oxidoreductase 1, EC:1.18.1.-

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of human spleen using 11404-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11404-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1/NR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 11404-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11404-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11404-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11404-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 11404-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11404-1-AP (NDOR1/NR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11404-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11404-1-AP (NDOR1/NR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11404-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDOR1/NR1 antibody (11404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 11404-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDOR1/NR1 antibody (11404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11404-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HaCaT cells, human liver tissue, human spleen tissue, human lung tissue, mouse thymus tissue, mouse testis tissue, HeLa cells, Jurkat cells, U-251 cells, NIH/3T3 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 1 publications below

Product Information

11404-1-AP targets NDOR1/NR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NDOR1/NR1 fusion protein Ag1909 相同性解析による交差性が予測される生物種
Full Name	NADPH dependent diflavin oxidoreductase 1
Calculated molecular weight	606 aa, 68 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC015735
Gene symbol	NDOR1
Gene ID (NCBI)	27158
RRID	AB_2251225
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NDOR1/NR1 antibody 11404-1-AP	Download protocol
IHC protocol for NDOR1/NR1 antibody 11404-1-AP	Download protocol
IF protocol for NDOR1/NR1 antibody 11404-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Life Sci Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction Authors - Yu Ma View Article
mouse	WB	ACS Chem Neurosci Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus. Authors - Lei Wu View Article
mouse	WB	Phytother Res Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission. Authors - Lei Wu View Article
mouse	WB	Toxicology PMID: 29800586 Authors - Xiaolong Wang View Article
mouse	WB	Behav Brain Res Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice Authors - Shukun Jiang View Article
human	WB	J Mol Neurosci PMID: 28730337 Authors - Xiaolong Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDOR1/NR1 Polyclonal antibody

NDOR1/NR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of human spleen using 11404-1-AP

WB analysis using 11404-1-AP

WB analysis of mouse thymus using 11404-1-AP

WB analysis of mouse testis using 11404-1-AP

WB analysis of human lung using 11404-1-AP

WB analysis of human liver using 11404-1-AP

IHC staining of mouse testis using 11404-1-AP

IHC staining of mouse testis using 11404-1-AP

IF Staining of HeLa using 11404-1-AP

IF Staining of HeLa using 11404-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.

PMID: 29800586

Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice

PMID: 28730337