Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
12015-1-AP targets NDRG2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NDRG2 fusion protein Ag2629 相同性解析による交差性が予測される生物種 |
| Full Name | NDRG family member 2 |
| Calculated molecular weight | 371 aa, 39 kDa |
| Observed molecular weight | 41-43 kDa |
| GenBank accession number | BC010458 |
| Gene Symbol | NDRG2 |
| Gene ID (NCBI) | 57447 |
| RRID | AB_10666856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UN36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NDRG2, or N-myc downstream-regulated gene 2, is a tumor suppressor gene that plays a significant role in cancer development and progression. It is associated with tumor incidence, progression, and metastasis, and is known to regulate tumor-associated genes. NDRG2 is part of the NDRG family, which includes NDRG1, NDRG2, NDRG3, and NDRG4. These proteins are characterized by an esterase/lipase/thioesterase active site serine and an α/β hydrolase fold of approximately 220 amino acids, although they do not exhibit enzyme activity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NDRG2 antibody 12015-1-AP | Download protocol |
| IHC protocol for NDRG2 antibody 12015-1-AP | Download protocol |
| IP protocol for NDRG2 antibody 12015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression | ||
J Ovarian Res NDRG2 gene expression pattern in ovarian cancer and its specific roles in inhibiting cancer cell proliferation and suppressing cancer cell apoptosis. |