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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDRG2 Monoclonal antibody

NDRG2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse, Pig and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E3F4

Cat no : 67191-1-Ig

Synonyms

DKFZp781G1938, FLJ25522, KIAA1248, NDRG family member 2, NDRG2, Protein NDRG2, Protein Syld709613, SYLD

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NDRG2 Monoclonal antibody (67191-1-Ig)

    WB analysis using 67191-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67191-1-Ig (NDRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDRG2 Monoclonal antibody (67191-1-Ig)

    IHC staining of mouse brain using 67191-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67191-1-Ig (NDRG2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDRG2 Monoclonal antibody (67191-1-Ig)

    IHC staining of mouse brain using 67191-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67191-1-Ig (NDRG2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NDRG2 Monoclonal antibody (67191-1-Ig)

    IHC staining of mouse cerebellum using 67191-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67191-1-Ig (NDRG2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NDRG2 Monoclonal antibody (67191-1-Ig)

    IHC staining of mouse cerebellum using 67191-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67191-1-Ig (NDRG2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman heart tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67191-1-Ig targets NDRG2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.

Tested Reactivity Human, Rat, Mouse, Pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NDRG2 fusion protein Ag28612 相同性解析による交差性が予測される生物種
Full Name NDRG family member 2
Calculated molecular weight 371 aa, 39 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC010458
Gene symbol NDRG2
Gene ID (NCBI) 57447
RRIDAB_2882486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDRG2 antibody 67191-1-IgDownload protocol
IHC protocol for NDRG2 antibody 67191-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage

Authors - Dayun Feng
View Article