Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, mouse kidney tissue |
| Positive IHC detected in | human medulloblastoma tissue, human colon tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12184-1-AP targets NDRG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NDRG4 fusion protein Ag3029 相同性解析による交差性が予測される生物種 |
| Full Name | NDRG family member 4 |
| Calculated molecular weight | 339 aa, 37 kDa |
| Observed molecular weight | 37 kDa, 38 kDa and 41 kDa |
| GenBank accession number | BC011795 |
| Gene Symbol | NDRG4 |
| Gene ID (NCBI) | 65009 |
| RRID | AB_2235852 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NDRG4 antibody 12184-1-AP | Download protocol |
| IHC protocol for NDRG4 antibody 12184-1-AP | Download protocol |
| IF protocol for NDRG4 antibody 12184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
DNA Cell Biol The Role of N-myc Downstream-Regulated Gene Family in Glioma Based on Bioinformatics Analysis. | ||
BMB Rep Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function. | ||
Cell Rep The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. |