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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFA2 Polyclonal antibody

NDUFA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15889-1-AP

Synonyms

B8, CI B8, Complex I B8, NDUFA2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA2 Polyclonal antibody (15889-1-AP)

    WB analysis using 15889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15889-1-AP (NDUFA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFA2 Polyclonal antibody (15889-1-AP)

    IHC staining of mouse heart using 15889-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15889-1-AP (NDUFA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFA2 Polyclonal antibody (15889-1-AP)

    IHC staining of mouse heart using 15889-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15889-1-AP (NDUFA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15889-1-AP targets NDUFA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA2 fusion protein Ag8675 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
Calculated molecular weight 99 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003674
Gene symbol NDUFA2
Gene ID (NCBI) 4695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFA2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2), also known as NADH ubiquinone oxidoreductase B8 subunit, is one of accessory subunits of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). NDUFS2 is vital for growth, ROS generation, membrane integrity, apoptosis, and mitochondrial energetics.

Protocols

Product Specific Protocols
WB protocol for NDUFA2 antibody 15889-1-APDownload protocol
IHC protocol for NDUFA2 antibody 15889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols