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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFA3 Polyclonal antibody

NDUFA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17257-1-AP

Synonyms

NADH-ubiquinone oxidoreductase B9 subunit, Complex I-B9, CI-B9, CIB9, CI B9

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    WB analysis of human brain using 17257-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDUFA3 Polyclonal antibody (17257-1-AP)

    WB analysis of HeLa using 17257-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    IHC staining of human liver using 17257-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    IHC staining of human stomach using 17257-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    IHC staining of human liver using 17257-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17257-1-AP targets NDUFA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA3 fusion protein Ag10912 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa
Calculated molecular weight65aa,7 kDa; 84aa,9 kDa
Observed molecular weight 9 kDa
GenBank accession numberBC011021
Gene symbol NDUFA3
Gene ID (NCBI) 4696
RRIDAB_2150631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFA3 antibody 17257-1-APDownload protocol
IHC protocol for NDUFA3 antibody 17257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
View Article
humanWB

J Biol Chem

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Authors - Yanchun Ji
View Article
humanWB

Invest Ophthalmol Vis Sci

Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy.

Authors - Juanjuan Zhang
View Article