• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NDUFA4L2 Polyclonal antibody

NDUFA4L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16480-1-AP

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    WB analysis using 16480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    WB analysis of HeLa using 16480-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IP experiment of HeLa using 16480-1-AP

    IP result of anti-NDUFA4L2 (IP:16480-1-AP, 4ug; Detection:16480-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human liver cancer using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human liver cancer using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human kidney using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of mouse kidney using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human kidney using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of mouse kidney using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human liver using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human liver using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human kidney using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IHC staining of human kidney using 16480-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFA4L2 Polyclonal antibody (16480-1-AP)

    IF Staining of HeLa using 16480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (16480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HUVEC cells, HepG2 cells, Jurkat clells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16480-1-AP targets NDUFA4L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA4L2 fusion protein Ag9600 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC011910
Gene symbol NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_2150637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFA4L2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Protocols

Product Specific Protocols
WB protocol for NDUFA4L2 antibody 16480-1-APDownload protocol
IHC protocol for NDUFA4L2 antibody 16480-1-APDownload protocol
IF protocol for NDUFA4L2 antibody 16480-1-APDownload protocol
IP protocol for NDUFA4L2 antibody 16480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Adv Sci (Weinh)

Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma

Authors - Yongxu Jia
View Article
humanWB

Cell Metab

Induction of the mitochondrial NDUFA4L2 protein by HIF-1α decreases oxygen consumption by inhibiting Complex I activity.

Authors - Daniel Tello
  • KD Validated
View Article
humanWB,IHC,IF,IP

Ther Adv Med Oncol

NDUFA4L2 promotes trastuzumab resistance in HER2-positive breast cancer.

Authors - Yuan Yuan
View Article
mouseIF

Sci Signal

Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.

Authors - Alejandro Moreno-Domínguez
  • KO Validated
View Article
ratWB

Basic Res Cardiol

Metabolic adaptation to chronic hypoxia in cardiac mitochondria.

Authors - Heather Lisa C LC
View Article
human,ratWB

Nutrients

Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy

Authors - Yujie Zhong
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Josh (Verified Customer) (10-28-2021)

Clear, good signal at expected MW (10kDa) by western blot. Non specific bands at 50 and 70 kDa (and to a lesser extent, 15 and 100 kDa).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: clear cell renal cell carcinoma cell line RCC4
NDUFA4L2 Antibody Western Blot validation (1:1000 dilution) in clear cell renal cell carcinoma cell line RCC4 (Cat no:16480-1-AP)
FH

Daniel (Verified Customer) (09-30-2019)

Kidney cancer mouse model stained for Ndufa4l2 (HRP detection by DAB stain).

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney (TRACK model)
NDUFA4L2 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16480-1-AP)