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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA4L2 Recombinant antibody (84498-1-RR)

    WB analysis using 84498-1-RR (same clone as 84498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NDUFA4L2 Recombinant antibody (84498-1-RR)

    IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NDUFA4L2 Recombinant antibody (84498-1-RR)

    IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFA4L2 Recombinant antibody (84498-1-RR)

    IF Staining of HeLa using 84498-1-RR (same clone as 84498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (84498-1-RR, Clone: 241622B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84498-1-RR

    Affinity and Kinetic Characterization of 84498-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84498-1-RR against Human NDUFA4L2 were performed. The affinity constant is below 1 pM.

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NDUFA4L2 Recombinant antibody, PBS Only

NDUFA4L2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84498-1-PBS
Clone No.241622B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84498-1-PBS targets NDUFA4L2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFA4L2 fusion protein Ag9600 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NRX3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFA4L2 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.