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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFA9 Polyclonal antibody, PBS Only

NDUFA9 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29621-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA9 Polyclonal antibody (29621-1-AP)

    WB analysis using 29621-1-AP (same clone as 29621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29621-1-AP (NDUFA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using NDUFA9 Polyclonal antibody (29621-1-AP)

    IHC staining of human hepatocirrhosis using 29621-1-AP (same clone as 29621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29621-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using NDUFA9 Polyclonal antibody (29621-1-AP)

    IHC staining of human hepatocirrhosis using 29621-1-AP (same clone as 29621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29621-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29621-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29621-1-PBS targets NDUFA9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA9 fusion protein Ag31222 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight35 kDa
GenBank accession numberBC009311
Gene symbol NDUFA9
Gene ID (NCBI) 4704
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFA9 (NADH: ubiquinone oxidoreductase subunit A9), also named as NDUFS2L, belongs to the complex I NDUFA9 subunit family. NDUFA9 gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Instead of being implicated in the catalysis, NDUFA9 is required for proper assembly of the complex I (PMID: 34938809). The predicted molecular weight of NDUFA9 is 42 kDa. With modification, the MW of NDUFA9 will be migrated to 35 kDa.