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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA9 Polyclonal antibody (29621-1-AP)

    WB analysis using 29621-1-AP (same clone as 29621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29621-1-AP (NDUFA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using NDUFA9 Polyclonal antibody (29621-1-AP)

    IHC staining of human hepatocirrhosis using 29621-1-AP (same clone as 29621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29621-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using NDUFA9 Polyclonal antibody (29621-1-AP)

    IHC staining of human hepatocirrhosis using 29621-1-AP (same clone as 29621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29621-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29621-1-PBS in a different storage buffer formulation.

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NDUFA9 Polyclonal antibody, PBS Only

NDUFA9 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29621-1-PBS targets NDUFA9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA9 fusion protein Ag31222 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight35 kDa
GenBank accession numberBC009311
Gene Symbol NDUFA9
Gene ID (NCBI) 4704
RRIDAB_2918331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16795
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFA9 (NADH: ubiquinone oxidoreductase subunit A9), also named as NDUFS2L, belongs to the complex I NDUFA9 subunit family. NDUFA9 gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Instead of being implicated in the catalysis, NDUFA9 is required for proper assembly of the complex I (PMID: 34938809). The predicted molecular weight of NDUFA9 is 42 kDa. With modification, the MW of NDUFA9 will be migrated to 35 kDa.