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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFAF3 Polyclonal antibody

NDUFAF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25621-1-AP

Synonyms

NDUF3, E3 3, C3orf60, 2P1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFAF3 Polyclonal antibody (25621-1-AP)

    WB analysis using 25621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25621-1-AP (NDUFAF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IHC staining of human ovary cancer using 25621-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25621-1-AP (NDUFAF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IF Staining of HeLa using 25621-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFAF3 antibody (25621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25621-1-AP targets NDUFAF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFAF3 fusion protein Ag22514 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight17 kDa
GenBank accession numberBC002873
Gene symbol NDUFAF3
Gene ID (NCBI) 25915
RRIDAB_3669488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404).

Protocols

Product Specific Protocols
WB protocol for NDUFAF3 antibody 25621-1-APDownload protocol
IHC protocol for NDUFAF3 antibody 25621-1-APDownload protocol
IF protocol for NDUFAF3 antibody 25621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols