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  • Featured Product
  • KD/KO Validated

NDUFB10 Polyclonal antibody

NDUFB10 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15589-1-AP

Synonyms

PDSW, NADH-ubiquinone oxidoreductase PDSW subunit, Complex I-PDSW, Complex I PDSW, CI-PDSW

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of HepG2 using 15589-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of human skeletal muscle using 15589-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of mouse liver using 15589-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of HepG2 using 15589-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of HepG2 using 15589-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of mouse liver using 15589-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of mouse liver using 15589-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of mouse liver using 15589-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    WB analysis of mouse liver using 15589-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NDUFB10 Polyclonal antibody (15589-1-AP)

    IP experiment of HepG2 using 15589-1-AP

    IP result of anti-NDUFB10 (IP:15589-1-AP, 4ug; Detection:15589-1-AP 1:1000) with HepG2 cells lysate 1000ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human ovary cancer using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human kidney using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human kidney using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human ovary using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human heart using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human heart using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human kidney using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human kidney using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human placenta using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human placenta using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human skin using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human skin using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human brain using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human brain using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human spleen using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using NDUFB10 Polyclonal antibody (15589-1-AP)

    IHC staining of human spleen using 15589-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHepG2 cells, human skeletal muscle tissue, mouse liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15589-1-AP targets NDUFB10 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB10 fusion protein Ag7922 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005829
Gene symbol NDUFB10
Gene ID (NCBI) 4716
RRIDAB_2150790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFB10(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10) is also named as PDSW and belongs to the complex I NDUFB10 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. The Complex I functions in the transfer of electrons from NADH to the respiratory chain.

Protocols

Product Specific Protocols
WB protocol for NDUFB10 antibody 15589-1-APDownload protocol
IHC protocol for NDUFB10 antibody 15589-1-APDownload protocol
IP protocol for NDUFB10 antibody 15589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Stem Cell

LIN28 Regulates Stem Cell Metabolism and Conversion to Primed Pluripotency.

Authors - Jin Zhang
View Article
humanWB

Nat Cell Biol

LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.

Authors - Kaloyan M Tsanov
View Article
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
View Article
humanWB

Proc Natl Acad Sci U S A

Assembly factors for the membrane arm of human complex I.

Authors - Andrews Byron B
View Article
humanWB

Cell Rep

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Authors - Timothy D Martin
  • KO Validated
View Article
mouseWB

Front Endocrinol (Lausanne)

GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging.

Authors - Bronwen Martin
View Article