Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HepG2 using 15589-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 15589-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15589-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15589-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15589-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15589-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15589-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15589-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15589-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15589-1-AP
IP result of anti-NDUFB10 (IP:15589-1-AP, 4ug; Detection:15589-1-AP 1:1000) with HepG2 cells lysate 1000ug.
IHC staining of human ovary cancer using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 15589-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

NDUFB10 Polyclonal antibody

NDUFB10 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15589-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PDSW, NADH-ubiquinone oxidoreductase PDSW subunit, Complex I-PDSW, Complex I PDSW, CI-PDSW

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, human skeletal muscle tissue, mouse liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below

Product Information

15589-1-AP targets NDUFB10 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NDUFB10 fusion protein Ag7922 相同性解析による交差性が予測される生物種
Full Name	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC005829
Gene Symbol	NDUFB10
Gene ID (NCBI)	4716
RRID	AB_2150790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O96000
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NDUFB10(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10) is also named as PDSW and belongs to the complex I NDUFB10 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. The Complex I functions in the transfer of electrons from NADH to the respiratory chain.

Protocols

Product Specific Protocols
WB protocol for NDUFB10 antibody 15589-1-AP	Download protocol
IHC protocol for NDUFB10 antibody 15589-1-AP	Download protocol
IP protocol for NDUFB10 antibody 15589-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell LIN28 Regulates Stem Cell Metabolism and Conversion to Primed Pluripotency. Authors - Jin Zhang View Article
human	WB	Nat Cell Biol LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency. Authors - Kaloyan M Tsanov View Article
human	WB	Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria Authors - Sabine Poerschke View Article
human	WB	Proc Natl Acad Sci U S A Assembly factors for the membrane arm of human complex I. Authors - Andrews Byron B View Article
human	WB	Cell Rep A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells. Authors - Timothy D Martin KO Validated View Article
mouse	WB	Front Endocrinol (Lausanne) GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging. Authors - Bronwen Martin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 15589-1-AP

WB analysis of human skeletal muscle using 15589-1-AP

WB analysis of mouse liver using 15589-1-AP

WB analysis of mouse liver using 15589-1-AP

WB analysis of mouse liver using 15589-1-AP

WB analysis of mouse liver using 15589-1-AP

WB analysis of HepG2 using 15589-1-AP

WB analysis of HepG2 using 15589-1-AP

WB analysis of mouse liver using 15589-1-AP

IP experiment of HepG2 using 15589-1-AP

IHC staining of human ovary cancer using 15589-1-AP

IHC staining of human ovary using 15589-1-AP

IHC staining of human kidney using 15589-1-AP

IHC staining of human kidney using 15589-1-AP

IHC staining of human heart using 15589-1-AP

IHC staining of human heart using 15589-1-AP

IHC staining of human kidney using 15589-1-AP

IHC staining of human kidney using 15589-1-AP

IHC staining of human placenta using 15589-1-AP

IHC staining of human placenta using 15589-1-AP

IHC staining of human skin using 15589-1-AP

IHC staining of human skin using 15589-1-AP

IHC staining of human brain using 15589-1-AP

IHC staining of human brain using 15589-1-AP

IHC staining of human spleen using 15589-1-AP

IHC staining of human spleen using 15589-1-AP

NDUFB10 Polyclonal antibody

NDUFB10 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LIN28 Regulates Stem Cell Metabolism and Conversion to Primed Pluripotency.

LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Assembly factors for the membrane arm of human complex I.

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging.