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NDUFB8 Polyclonal antibody
NDUFB8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14794-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, A549 cells, various samples, 3T3-L1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 47 publications below |
IHC | See 3 publications below |
IF | See 4 publications below |
Product Information
14794-1-AP targets NDUFB8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種 |
Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa |
Calculated molecular weight | 22 kDa |
Observed molecular weight | 19-22 kDa |
GenBank accession number | BC000466 |
Gene symbol | NDUFB8 |
Gene ID (NCBI) | 4714 |
RRID | AB_2150970 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NDUFB8 antibody 14794-1-AP | Download protocol |
IHC protocol for NDUFB8 antibody 14794-1-AP | Download protocol |
IF protocol for NDUFB8 antibody 14794-1-AP | Download protocol |
IP protocol for NDUFB8 antibody 14794-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4. | ||
Nat Commun Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength. | ||
J Exp Med FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics. | ||
Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. | ||
J Cachexia Sarcopenia Muscle MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism. | ||
Cell Death Dis TGR5 supresses cGAS/STING pathway by inhibiting GRP75-mediated endoplasmic reticulum-mitochondrial coupling in diabetic retinopathy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Edward (Verified Customer) (11-15-2022) | Used with 12 ug protein lysate NDUFB8 works well, both with ECL detection and fluorescent secondary antibodies it gives a strong band
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