Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 14794-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of various samples using 14794-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
WB analysis of A549 using 14794-1-AP
WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.
IP experiment of HepG2 using 14794-1-AP
IP result of anti-NDUFB8 (IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug.
IHC staining of human liver using 14794-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14794-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14794-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 14794-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 14794-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

NDUFB8 Polyclonal antibody

NDUFB8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14794-1-AP

Publications(57)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NADH-ubiquinone oxidoreductase ASHI subunit, Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (57)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, A549 cells, various samples, 3T3-L1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 53 publications below
IHC	See 3 publications below
IF	See 4 publications below

Product Information

14794-1-AP targets NDUFB8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種
Full Name	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight	22 kDa
Observed molecular weight	19-22 kDa
GenBank accession number	BC000466
Gene Symbol	NDUFB8
Gene ID (NCBI)	4714
RRID	AB_2150970
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95169
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 14794-1-AP	Download protocol
IHC protocol for NDUFB8 antibody 14794-1-AP	Download protocol
IF protocol for NDUFB8 antibody 14794-1-AP	Download protocol
IP protocol for NDUFB8 antibody 14794-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
canine	WB	Cell Metab Formation of I2+III2 supercomplex rescues respiratory chain defects Authors - Chao Liang View Article
mouse	WB	Sci Adv Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4. Authors - Qiqi Guo View Article
mouse	WB	Nat Commun Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength. Authors - Zhisheng Xu View Article
mouse	WB	J Exp Med FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics. Authors - Yujing Yin View Article
mouse	WB	Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. Authors - Xiao-Tao He View Article
pig	WB	J Cachexia Sarcopenia Muscle MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism. Authors - Ming Qi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14794-1-AP

WB analysis of various samples using 14794-1-AP

WB analysis of A549 using 14794-1-AP

IP experiment of HepG2 using 14794-1-AP

IHC staining of human liver using 14794-1-AP

IHC staining of human liver using 14794-1-AP

IHC staining of human heart using 14794-1-AP

IHC staining of human heart using 14794-1-AP

IF Staining of HepG2 using 14794-1-AP

NDUFB8 Polyclonal antibody

NDUFB8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Formation of I2+III2 supercomplex rescues respiratory chain defects

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics.

Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.

MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.