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  • Featured Product
  • KD/KO Validated

NDUFB8 Polyclonal antibody, PBS Only

NDUFB8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14794-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB8 Polyclonal antibody (14794-1-AP)

    WB analysis using 14794-1-AP (same clone as 14794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various samples using NDUFB8 Polyclonal antibody (14794-1-AP)

    WB analysis of various samples using 14794-1-AP (same clone as 14794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using NDUFB8 Polyclonal antibody (14794-1-AP)

    WB analysis of A549 using 14794-1-AP (same clone as 14794-1-PBS)

    WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NDUFB8 Polyclonal antibody (14794-1-AP)

    IP experiment of HepG2 using 14794-1-AP (same clone as 14794-1-PBS)

    IP result of anti-NDUFB8 (IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB8 Polyclonal antibody (14794-1-AP)

    IHC staining of human liver using 14794-1-AP (same clone as 14794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB8 Polyclonal antibody (14794-1-AP)

    IHC staining of human liver using 14794-1-AP (same clone as 14794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB8 Polyclonal antibody (14794-1-AP)

    IHC staining of human heart using 14794-1-AP (same clone as 14794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB8 Polyclonal antibody (14794-1-AP)

    IHC staining of human heart using 14794-1-AP (same clone as 14794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Polyclonal antibody (14794-1-AP)

    IF Staining of HepG2 using 14794-1-AP (same clone as 14794-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14794-1-PBS targets NDUFB8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.