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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFB8 Recombinant antibody

NDUFB8 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230510H1

Cat no : 83216-4-RR

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB8 Recombinant antibody (83216-4-RR)

    WB analysis using 83216-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-4-RR (NDUFB8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NDUFB8 Recombinant antibody (83216-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83216-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-4-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Recombinant antibody (83216-4-RR)

    IF Staining of HepG2 using 83216-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-4-RR, Clone: 230510H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using NDUFB8 Recombinant antibody (83216-4-RR)

    FC experiment of HeLa using 83216-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-4-RR, Clone:230510H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83216-4-RR

    Affinity and Kinetic Characterization of 83216-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-4-RR against Human NDUFB8 were performed. The affinity constant is 5.00 nM.

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Tested Applications

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, Jurkat cells, mouse liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83216-4-RR is NDUFB8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-4-RRDownload protocol
IHC protocol for NDUFB8 antibody 83216-4-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-4-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols