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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB8 Recombinant antibody (83216-4-RR)

    WB analysis using 83216-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-4-RR (NDUFB8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NDUFB8 Recombinant antibody (83216-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83216-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-4-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFB8 Recombinant antibody (83216-4-RR)

    IF Staining of HepG2 using 83216-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-4-RR, Clone: 230510H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using NDUFB8 Recombinant antibody (83216-4-RR)

    FC experiment of HeLa using 83216-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-4-RR, Clone:230510H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83216-4-RR

    Affinity and Kinetic Characterization of 83216-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-4-RR against Human NDUFB8 were performed. The affinity constant is 5.00 nM.

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NDUFB8 Recombinant antibody

NDUFB8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83216-4-RR
Clone No.230510H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, Jurkat cells, mouse liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83216-4-RR targets NDUFB8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC000466
Gene Symbol NDUFB8
Gene ID (NCBI) 4714
RRIDAB_3670902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-4-RRDownload protocol
IHC protocol for NDUFB8 antibody 83216-4-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-4-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols