NDUFC2 Polyclonal antibody

Name: NDUFC2 Polyclonal antibody
Brand: Proteintech
SKU: 15573-1-AP

NDUFC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15573-1-AP

Synonyms

HLC 1, Complex I-B14.5b, Complex I B14.5b, CI-B14.5b, CI B14.5b

Validation Data Gallery

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AllWBIHC

WB analysis using 15573-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15573-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15573-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15573-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 15573-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15573-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 15573-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human hepatocirrhosis using 15573-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 15573-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 15573-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 15573-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, human kidney tissue, human liver tissue, Hela cells, HepG2 cells, mouse brain tissue, mouse heart tissue, rat brain tissue
Positive IHC detected in	human kidney tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

15573-1-AP targets NDUFC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NDUFC2 fusion protein Ag7919 相同性解析による交差性が予測される生物種
Full Name	NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
Calculated molecular weight	14 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC007323
Gene symbol	NDUFC2
Gene ID (NCBI)	4718
RRID	AB_10666733
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFC2 antibody 15573-1-AP	Download protocol
IHC protocol for NDUFC2 antibody 15573-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function. Authors - Brooke C Wilson View Article
pig	WB	Sci Rep Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs. Authors - Liangliang Fu View Article
human	WB	Cell Rep A membrane arm of mitochondrial complex I sufficient to promote respirasome formation. Authors - Hezhi Fang View Article
mouse	WB	Environ Int Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury Authors - Xing Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFC2 Polyclonal antibody

NDUFC2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 15573-1-AP

WB analysis using 15573-1-AP

WB analysis of human liver using 15573-1-AP

WB analysis of human kidney using 15573-1-AP

IHC staining of human kidney using 15573-1-AP

IHC staining of human kidney using 15573-1-AP

IHC staining of human hepatocirrhosis using 15573-1-AP

IHC staining of human hepatocirrhosis using 15573-1-AP

IHC staining of human kidney using 15573-1-AP

IHC staining of human kidney using 15573-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury