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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFS2 Polyclonal antibody

NDUFS2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28125-1-AP

Synonyms

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS2 Polyclonal antibody (28125-1-AP)

    WB analysis using 28125-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28125-1-AP (NDUFS2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFS2 Polyclonal antibody (28125-1-AP)

    IF Staining of HepG2 using 28125-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS2 antibody (28125-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28125-1-AP targets NDUFS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFS2 fusion protein Ag27078 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 53 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC000170
Gene symbol NDUFS2
Gene ID (NCBI) 4720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFS2 antibody 28125-1-APDownload protocol
IF protocol for NDUFS2 antibody 28125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols