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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS2 Recombinant antibody (84880-1-RR)

    WB analysis using 84880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84880-1-RR (NDUFS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using NDUFS2 Recombinant antibody (84880-1-RR)

    FC experiment of HepG2 using 84880-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using NDUFS2 Recombinant antibody (84880-1-RR)

    FC experiment of HeLa using 84880-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84880-1-RR

    Affinity and Kinetic Characterization of 84880-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84880-1-RR against Human NDUFS2 were performed. The affinity constant is 1.61 nM.

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NDUFS2 Recombinant antibody

NDUFS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84880-1-RR
Clone No.242238D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD, CI 49kD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive FC (Intra) detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84880-1-RR targets NDUFS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDUFS2 fusion protein Ag28008 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 53 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC000170
Gene Symbol NDUFS2
Gene ID (NCBI) 4720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial Complex I, responsible for the oxidation of NADH, is suspected to be an integral component of the O2-sensing pathway. NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2), a key subunit of complex I in the mitochondrial electron transport chain (ETC), is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction (PMID: 30922174). Functions of NDUFS2 are associated with growth, cell-membrane integrity, ROS generation, apoptosis, necrosis, Complex I respiration, and synthesis of ATP (PMID: 33744462).

Protocols

Product Specific Protocols
WB protocol for NDUFS2 antibody 84880-1-RRDownload protocol
FC protocol for NDUFS2 antibody 84880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols