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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFS3 Monoclonal antibody

NDUFS3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1B3

Cat no : 68066-1-Ig

Synonyms

CI 30kD, Complex I 30kD, NDUFS3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS3 Monoclonal antibody (68066-1-Ig)

    WB analysis using 68066-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of pig brain tissue using NDUFS3 Monoclonal antibody (68066-1-Ig)

    WB analysis of pig brain using 68066-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit brain tissue using NDUFS3 Monoclonal antibody (68066-1-Ig)

    WB analysis of rabbit brain using 68066-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using NDUFS3 Monoclonal antibody (68066-1-Ig)

    WB analysis of rat brain using 68066-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NDUFS3 Monoclonal antibody (68066-1-Ig)

    WB analysis of mouse brain using 68066-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NDUFS3 Monoclonal antibody (68066-1-Ig)

    IHC staining of mouse testis using 68066-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NDUFS3 Monoclonal antibody (68066-1-Ig)

    IHC staining of mouse testis using 68066-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68066-1-Ig targets NDUFS3 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse, rat samples.

Tested Reactivity Human, pig, rabbit, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFS3 fusion protein Ag7072 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000617
Gene symbol NDUFS3
Gene ID (NCBI) 4722
RRIDAB_2918807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

Protocols

Product Specific Protocols
WB protocol for NDUFS3 antibody 68066-1-IgDownload protocol
IHC protocol for NDUFS3 antibody 68066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols