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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS5 Polyclonal antibody (15224-1-AP)

    WB analysis using 15224-1-AP (same clone as 15224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15224-1-AP (NDUFS5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NDUFS5 Polyclonal antibody (15224-1-AP)

    WB analysis of HEK-293 using 15224-1-AP (same clone as 15224-1-PBS)

    WB result of NDUFS5 antibody (15224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS5 transfected HEK-293 cells. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFS5 Polyclonal antibody (15224-1-AP)

    IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFS5 Polyclonal antibody (15224-1-AP)

    IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NDUFS5 Polyclonal antibody, PBS Only

NDUFS5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15224-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15224-1-PBS targets NDUFS5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFS5 fusion protein Ag7441 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 13 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001884
Gene Symbol NDUFS5
Gene ID (NCBI) 4725
RRIDAB_2149021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43920
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150).