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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NDUFS5 Monoclonal antibody (66053-1-Ig)

    WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using NDUFS5 Monoclonal antibody (66053-1-Ig)

    WB analysis of L02 using 66053-1-Ig (same clone as 66053-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using NDUFS5 Monoclonal antibody (66053-1-Ig)

    WB analysis of fetal human brain using 66053-1-Ig (same clone as 66053-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NDUFS5 Monoclonal antibody (66053-1-Ig)

    WB analysis of HEK-293 using 66053-1-Ig (same clone as 66053-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFS5 Monoclonal antibody (66053-1-Ig)

    IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFS5 Monoclonal antibody (66053-1-Ig)

    IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFS5 Monoclonal antibody (66053-1-Ig)

    IHC staining of human liver using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFS5 Monoclonal antibody (66053-1-Ig)

    IHC staining of human liver using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50607-1

    Cytometric bead array standard curve of MP50607-1

    Cytometric bead array standard curve of MP50607-1, NDUFS5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66053-2-PBS. Detection antibody: 66053-1-PBS. Standard:Ag7663. Range: 3.125-100 ng/mL.

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NDUFS5 Monoclonal antibody, PBS Only (Detector)

NDUFS5 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66053-1-PBS
Clone No.2C2A5

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, Complex I 15 kDa, CI-15 kDa, CI 15 kDa

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66053-1-PBS targets NDUFS5 as part of a matched antibody pair:

MP50607-1: 66053-2-PBS capture and 66053-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NDUFS5 fusion protein Ag7663 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 13 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001884
Gene Symbol NDUFS5
Gene ID (NCBI) 4725
RRIDAB_11043342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43920
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach's NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.