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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFS6 Monoclonal antibody, PBS Only

NDUFS6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Chicken

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4H3

Cat no : 68329-1-PBS

Synonyms

CI 13kD A, Complex I 13kD A, NDUFS6

Validation Data Gallery

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  • Western Blot (WB) analysis of rabbit heart tissue using NDUFS6 Monoclonal antibody (68329-1-Ig)

    WB analysis of rabbit heart using 68329-1-Ig (same clone as 68329-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NDUFS6 Monoclonal antibody (68329-1-Ig)

    WB analysis of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NDUFS6 Monoclonal antibody (68329-1-Ig)

    IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NDUFS6 Monoclonal antibody (68329-1-Ig)

    IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFS6 Monoclonal antibody (68329-1-Ig)

    IF Staining of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (68329-1-Ig, Clone: 2F4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68329-1-PBS targets NDUFS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFS6 fusion protein Ag6286 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 14 kDa
Observed molecular weight13-15 kDa
GenBank accession numberBC046155
Gene symbol NDUFS6
Gene ID (NCBI) 4726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.