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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFS8 Polyclonal antibody

NDUFS8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25172-1-AP

Synonyms

CI 23kD, Complex I 23kD, NDUFS8, TYKY, TYKY subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NDUFS8 Polyclonal antibody (25172-1-AP)

    WB analysis of mouse brain using 25172-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25172-1-AP (NDUFS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using NDUFS8 Polyclonal antibody (25172-1-AP)

    WB analysis of mouse heart using 25172-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25172-1-AP (NDUFS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFS8 Polyclonal antibody (25172-1-AP)

    IHC staining of human liver cancer using 25172-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFS8 Polyclonal antibody (25172-1-AP)

    IHC staining of human liver cancer using 25172-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25172-1-AP is NDUFS8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFS8 fusion protein Ag18531 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC119754
Gene symbol NDUFS8
Gene ID (NCBI) 4728
RRIDAB_2879939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFS8 antibody 25172-1-APDownload protocol
IHC protocol for NDUFS8 antibody 25172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

Combination of RNA-seq and proteomics reveals the mechanism of DL0410 treatment in APP/PS1 transgenic mouse model of Alzheimer's disease

Authors - Baoyue Zhang
View Article