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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFV2 Monoclonal antibody, PBS Only

NDUFV2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig, Chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4A5

Cat no : 68150-1-PBS

Synonyms

NDUFV2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFV2 Monoclonal antibody (68150-1-Ig)

    WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of chicken heart tissue using NDUFV2 Monoclonal antibody (68150-1-Ig)

    WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

    chicken heart tissue were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NDUFV2 Monoclonal antibody (68150-1-Ig)

    WB analysis of HeLa using 68150-1-Ig (same clone as 68150-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFV2 Monoclonal antibody (68150-1-Ig)

    IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFV2 Monoclonal antibody (68150-1-Ig)

    IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68150-1-PBS targets NDUFV2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFV2 fusion protein Ag7760 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Calculated molecular weight 27 kDa
Observed molecular weight24-27 kDa
GenBank accession numberBC001632
Gene symbol NDUFV2
Gene ID (NCBI) 4729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).