NDUFV3 Polyclonal antibody

NDUFV3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13430-1-AP

Synonyms

Renal carcinoma antigen NY-REN-4, NADH-ubiquinone oxidoreductase 9 kDa subunit, Complex I-9kD, Complex I 9kD, CI-9kD


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Tested Applications

Positive WB detected inHepG2 cells, human heart tissue, human liver tissue
Positive IHC detected inhuman placenta tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

13430-1-AP targets NDUFV3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFV3 fusion protein Ag4234 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa
Calculated molecular weight 108 aa, 12 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC033766
Gene symbol NDUFV3
Gene ID (NCBI) 4731
RRIDAB_2282462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFV3(NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial) is also named as CI-9kD,Renal carcinoma antigen NY-REN-4 and belongs to the complex I NDUFV3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Protocols

Product Specific Protocols
WB protocol for NDUFV3 antibody 13430-1-APDownload protocol
IHC protocol for NDUFV3 antibody 13430-1-APDownload protocol
IF protocol for NDUFV3 antibody 13430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.

Authors - Villeneuve Lance L
humanWB

J Pharmacol Exp Ther

Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.

Authors - Rui Ju
humanWB

FEBS Lett

A novel isoform of the human mitochondrial complex I subunit NDUFV3.

Authors - Marris G Dibley
humanWB

F1000Res

Androgen-dependent alternative mRNA isoform expression in prostate cancer cells.

Authors - Jennifer Munkley
mouseWB

Nat Struct Mol Biol

SCAF1 drives the compositional diversity of mammalian respirasomes

Authors - Irene Vercellino