Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21497-1-AP targets NEBL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NEBL fusion protein Ag15882 相同性解析による交差性が予測される生物種 |
| Full Name | nebulette |
| Calculated molecular weight | 1014 aa, 116 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC110453 |
| Gene Symbol | NEBL |
| Gene ID (NCBI) | 10529 |
| RRID | AB_3085659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76041 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nebulette, also known as NEBL, is a cardiac-specific isoform belonging to the nebulin family of proteins. Nebulette has 23 modular repeat structures of 35 amino acid residues each. Nebulette has an acidic region with unknown structure at its N-terminus, and a serine-rich region adjacent to an SH3 domain at its C-terminus (PMID: 2037050, 20951588). Nebulette functionally links sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Nebulette is abundantly expressed in cardiac muscle at Z-disc regions, but not in skeletal or smooth muscle.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEBL antibody 21497-1-AP | Download protocol |
| IHC protocol for NEBL antibody 21497-1-AP | Download protocol |
| IF protocol for NEBL antibody 21497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |