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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NECAB3 Polyclonal antibody

NECAB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18940-1-AP

Synonyms

APBA2BP, EFCBP3, NECAB3, Nek2 interacting protein 1, NIP1, STIP3, SYTIP2, X11L binding protein 51, XB51

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    WB analysis of human brain using 18940-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    WB analysis of human brain using 18940-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    WB analysis of rat brain using 18940-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    IHC staining of human pancreas using 18940-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    IHC staining of human pancreas using 18940-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

18940-1-AP targets NECAB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NECAB3 fusion protein Ag13493 相同性解析による交差性が予測される生物種
Full Name N-terminal EF-hand calcium binding protein 3
Calculated molecular weight362aa,41 kDa; 396aa,44 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC047673
Gene symbol NECAB3
Gene ID (NCBI) 63941
RRIDAB_10596622
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NECAB3, also named as APBA2BP, NIP1, SYTIP2 and XB51, is a potential substrate of Nek2. NECAB3 inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP). NECAB3 has three isoforms with MW 40 kDa, 44 kDa and 22 kDa.

Protocols

Product Specific Protocols
WB protocol for NECAB3 antibody 18940-1-APDownload protocol
IHC protocol for NECAB3 antibody 18940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

PLoS One

Amyloidosis, Synucleinopathy, and Prion Encephalopathy in a Neuropathic Lysosomal Storage Disease: The CNS-Biomarker Potential of Peripheral Blood.

Authors - Naughton Bartholomew J BJ
View Article