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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NECAP2 Polyclonal antibody

NECAP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15899-1-AP

Synonyms

NECAP 2, NECAP endocytosis associated 2, NECAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using NECAP2 Polyclonal antibody (15899-1-AP)

    WB analysis of MCF-7 using 15899-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NECAP2 Polyclonal antibody (15899-1-AP)

    WB analysis of HeLa using 15899-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using NECAP2 Polyclonal antibody (15899-1-AP)

    WB analysis of mouse cerebellum using 15899-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using NECAP2 Polyclonal antibody (15899-1-AP)

    WB analysis of human liver using 15899-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using NECAP2 Polyclonal antibody (15899-1-AP)

    WB analysis of human liver using 15899-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, human liver tissue, mouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15899-1-AP targets NECAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NECAP2 fusion protein Ag8721 相同性解析による交差性が予測される生物種
Full Name NECAP endocytosis associated 2
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC018914
Gene symbol NECAP2
Gene ID (NCBI) 55707
RRIDAB_2149187
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NECAP2 antibody 15899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oxid Med Cell Longev

Comprehensive Prognostic Analysis of Immune Implication Value and Oxidative Stress Significance of NECAP2 in Low-Grade Glioma

Authors - Zhichao Lu
View Article