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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD1 Polyclonal antibody, PBS Only

NEDD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13993-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis using 13993-1-AP (same clone as 13993-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis of human brain using 13993-1-AP (same clone as 13993-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis of SH-SY5Y using 13993-1-AP (same clone as 13993-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NEDD1 Polyclonal antibody (13993-1-AP)

    IP experiment of HeLa using 13993-1-AP (same clone as 13993-1-PBS)

    IP result of anti-NEDD1 (IP:13993-1-AP, 3ug; Detection:13993-1-AP 1:800) with HeLa cells lysate 2150ug. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13993-1-PBS targets NEDD1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD1 fusion protein Ag5077 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 1
Calculated molecular weight 72 kDa
Observed molecular weight 75 kDa, 62 kDa
GenBank accession numberBC027605
Gene symbol NEDD1
Gene ID (NCBI) 121441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.