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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD1 Polyclonal antibody, PBS Only

NEDD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13993-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis using 13993-1-AP (same clone as 13993-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis of human brain using 13993-1-AP (same clone as 13993-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis of SH-SY5Y using 13993-1-AP (same clone as 13993-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NEDD1 Polyclonal antibody (13993-1-AP)

    IP experiment of HeLa using 13993-1-AP (same clone as 13993-1-PBS)

    IP result of anti-NEDD1 (IP:13993-1-AP, 3ug; Detection:13993-1-AP 1:800) with HeLa cells lysate 2150ug. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13993-1-PBS targets NEDD1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD1 fusion protein Ag5077 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 1
Calculated molecular weight 72 kDa
Observed molecular weight 75 kDa, 62 kDa
GenBank accession numberBC027605
Gene symbol NEDD1
Gene ID (NCBI) 121441
RRIDAB_2149202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.