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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD4 Recombinant antibody, PBS Only (Detector)

NEDD4 Recombinant Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230478D8

Cat no : 83112-4-PBS

Synonyms

KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD4 Recombinant antibody (83112-4-RR)

    WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-4-RR (NEDD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NEDD4 Recombinant antibody (83112-4-RR)

    WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-4-RR (NEDD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NEDD4 Recombinant antibody (83112-4-RR)

    IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-4-RR, Clone: 230478D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NEDD4 Recombinant antibody (83112-4-RR)

    IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-4-RR, Clone: 230478D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve ofMP00234-1, NEDD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83112-2-PBS. Detection antibody: 83112-4-PBS. Standard: Ag16291. Range: 1.25-20 ng/mL.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83112-4-PBS targets NEDD4 as part of a matched antibody pair:

MP00234-1: 83112-2-PBS capture and 83112-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEDD4 fusion protein Ag16291 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 4
Calculated molecular weight 1319 aa, 149 kDa
GenBank accession numberBC152452
Gene symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.