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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD4L Monoclonal antibody

NEDD4L Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6H6

Cat no : 67276-1-Ig

Synonyms

KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of Jurkat using 67276-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of HSC-T6 using 67276-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of NIH/3T3 using 67276-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of 4T1 using 67276-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    IF Staining of PC-3 using 67276-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    IF Staining of Jurkat using 67276-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inPC-3 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67276-1-Ig targets NEDD4L in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEDD4L fusion protein Ag4565 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Calculated molecular weight 854 aa, 96 kDa
Observed molecular weight 112 kDa, 125 kDa
GenBank accession numberBC032597
Gene symbol NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2882545
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Protocols

Product Specific Protocols
WB protocol for NEDD4L antibody 67276-1-IgDownload protocol
IF protocol for NEDD4L antibody 67276-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols