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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of Jurkat using 67276-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of HSC-T6 using 67276-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of NIH/3T3 using 67276-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    WB analysis of 4T1 using 67276-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    IF Staining of Jurkat using 67276-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using NEDD4L Monoclonal antibody (67276-1-Ig)

    IF Staining of PC-3 using 67276-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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NEDD4L Monoclonal antibody

NEDD4L Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67276-1-Ig
Clone No.2F6H6
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inPC-3 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67276-1-Ig targets NEDD4L in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEDD4L fusion protein Ag4565 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Calculated molecular weight 854 aa, 96 kDa
Observed molecular weight 112 kDa, 125 kDa
GenBank accession numberBC032597
Gene Symbol NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2882545
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96PU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Protocols

Product Specific Protocols
WB protocol for NEDD4L antibody 67276-1-IgDownload protocol
IF protocol for NEDD4L antibody 67276-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Biochim Biophys Acta Mol Cell Res

NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras

Authors - Guozhu Chen
View Article
humanWB

Cell Biol Toxicol

Exosomal NEDD4L derived from HG+oxLDL-induced vascular endothelial cells accelerates macrophage M1 polarization and oxLDL uptake by ubiquitinating IκBα and PPARγ

Authors - Guozhu Chen
View Article