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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEDD9 Recombinant antibody, PBS Only (Capture)

NEDD9 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240310F8

Cat no : 83362-2-PBS

Synonyms

HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD9 Recombinant antibody (83362-2-RR)

    WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NEDD9 Recombinant antibody (83362-2-RR)

    WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NEDD9 Recombinant antibody (83362-2-RR)

    IF Staining of A549 using 83362-2-RR (same clone as 83362-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NEDD9 Recombinant antibody (83362-2-RR)

    IF Staining of MCF-7 using 83362-2-RR (same clone as 83362-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00378-2

    Cytometric bead array standard curve of MP00378-2

    Cytometric bead array standard curve of MP00378-2, NEDD9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83362-2-PBS. Detection antibody: 83362-4-PBS. Standard: Ag34170. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83362-2-PBS

    Affinity and Kinetic Characterization of 83362-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83362-2-PBS against Human NEDD9 was performed. The affinity constant is 0.225 nM.

  • Affinity and Kinetic Characterization of 83362-2-RR

    Affinity and Kinetic Characterization of 83362-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83362-2 against Human NEDD9 were performed. The affinity constant is 0.173 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83362-2-PBS targets NEDD9 as part of a matched antibody pair:

MP00378-2: 83362-2-PBS capture and 83362-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEDD9 fusion protein Ag34170 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 9
Calculated molecular weight 834 aa, 93 kDa
Observed molecular weight 105 kDa, 115 kDa
GenBank accession numberBC040207
Gene symbol NEDD9
Gene ID (NCBI) 4739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).