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NEIL3 Polyclonal antibody
NEIL3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, FC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11621-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IP | See 3 publications below |
| FC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
11621-1-AP targets NEIL3 in WB, IP, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NEIL3 fusion protein Ag2200 相同性解析による交差性が予測される生物種 |
| Full Name | nei endonuclease VIII-like 3 (E. coli) |
| Calculated molecular weight | 605 aa, 68 kDa |
| Observed molecular weight | 62-68 kDa |
| GenBank accession number | BC025954 |
| Gene symbol | NEIL3 |
| Gene ID (NCBI) | 55247 |
| RRID | AB_2251229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEIL3 antibody 11621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. | ||
Nucleic Acids Res Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.
| ||
EMBO J PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. | ||
Sci Rep NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells | ||
DNA Repair (Amst) Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway. | ||
Cell Rep NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jia (Verified Customer) (09-19-2019) | Good antibody for detecting NEIL3 in human cells.
|
