Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HL-60 cells, HeLa cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12710-1-AP targets NEK11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NEK11 fusion protein Ag3392 相同性解析による交差性が予測される生物種 |
| Full Name | NIMA (never in mitosis gene a)- related kinase 11 |
| Calculated molecular weight | 54 kDa, 74 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC028587 |
| Gene Symbol | NEK11 |
| Gene ID (NCBI) | 79858 |
| RRID | AB_2150345 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NG66 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NEK11(Serine/threonine-protein kinase) belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses.It controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.It has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEK11 antibody 12710-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |