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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEK11 Polyclonal antibody

NEK11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12710-1-AP

Synonyms

NEK11, NimA related protein kinase 11

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NEK11 Polyclonal antibody (12710-1-AP)

    WB analysis of HeLa using 12710-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NEK11 Polyclonal antibody (12710-1-AP)

    WB analysis of HeLa using 12710-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12710-1-AP targets NEK11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK11 fusion protein Ag3392 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)- related kinase 11
Calculated molecular weight 54 kDa, 74 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC028587
Gene symbol NEK11
Gene ID (NCBI) 79858
RRIDAB_2150345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEK11(Serine/threonine-protein kinase) belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses.It controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NEK11 antibody 12710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols