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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEK2 Polyclonal antibody

NEK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14233-1-AP

Synonyms

NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEK2 Polyclonal antibody (14233-1-AP)

    WB analysis using 14233-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NEK2 Polyclonal antibody (14233-1-AP)

    WB analysis of K-562 using 14233-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NEK2 Polyclonal antibody (14233-1-AP)

    WB analysis of K-562 using 14233-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NEK2 Polyclonal antibody (14233-1-AP)

    IHC staining of mouse testis using 14233-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14233-1-AP (NEK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using NEK2 Polyclonal antibody (14233-1-AP)

    IHC staining of human skin cancer using 14233-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14233-1-AP (NEK2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NEK2 Polyclonal antibody (14233-1-AP)

    IF Staining of HeLa using 14233-1-AP

    Immunofluorescent analysis of Hela cells, using NEK2 antibody 14233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHEK-293T cells, K-562 cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14233-1-AP targets NEK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK2 fusion protein Ag5474 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)-related kinase 2
Calculated molecular weight 52 kDa
Observed molecular weight 45-52 kDa
GenBank accession numberBC043502
Gene symbol NEK2
Gene ID (NCBI) 4751
RRIDAB_2150373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NEK2 antibody 14233-1-APDownload protocol
IHC protocol for NEK2 antibody 14233-1-APDownload protocol
IF protocol for NEK2 antibody 14233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article
humanWB

Nucleic Acids Res

The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation

Authors - Yilin Wang
View Article