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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEK3 Polyclonal antibody

NEK3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12843-1-AP

Synonyms

NimA-related protein kinase 3, NimA related protein kinase 3, Never in mitosis A-related kinase 3, HSPK36, HSPK 36

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NEK3 Polyclonal antibody (12843-1-AP)

    WB analysis of SH-SY5Y using 12843-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12843-1-AP (NEK3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NEK3 Polyclonal antibody (12843-1-AP)

    IF Staining of SH-SY5Y using 12843-1-AP

    Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 12843-1-AP (NEK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12843-1-AP targets NEK3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK3 fusion protein Ag3856 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)-related kinase 3
Calculated molecular weight 506 aa, 58 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC019916
Gene symbol NEK3
Gene ID (NCBI) 4752
RRIDAB_2877888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NEK3 antibody 12843-1-APDownload protocol
IF protocol for NEK3 antibody 12843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen
View Article