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NEK6 Polyclonal antibody

Name: NEK6 Polyclonal antibody
Brand: Proteintech
SKU: 10378-1-AP

NEK6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10378-1-AP

Synonyms

Protein kinase SID6-1512, Protein kinase SID6 1512, NimA-related protein kinase 6, NimA related protein kinase 6, Never in mitosis A-related kinase 6

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis using 10378-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10378-1-AP
WB result of NEK6 antibody (10378-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK6 transfected HEK-293 cells.
WB analysis of HT-1080 using 10378-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10378-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 10378-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10378-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 10378-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 10378-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10378-1-AP (NEK6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10378-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HT-1080 cells, HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

10378-1-AP targets NEK6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NEK6 fusion protein Ag0266 相同性解析による交差性が予測される生物種
Full Name	NIMA (never in mitosis gene a)-related kinase 6
Calculated molecular weight	35 kDa
Observed molecular weight	35-45 kDa
GenBank accession number	BC000101
Gene symbol	NEK6
Gene ID (NCBI)	10783
RRID	AB_2251272
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Aspergillus nidulans 'never in mitosis A' (NIMA) is a serine/ threonine kinase that controls initiation of mitosis, whereas its inactivation is necessary for mitotic exit. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. Human NIMA-related kinase 6 (NEK6, synonym: SID6-1512) is comprised of 338 amino acids and shows both nuclear and cytoplasmic localizations in HeLa cells. NEK6 is required for mitotic progression of human cells. NEK6 is phosphorylated and activated during M phase. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis. Recombinant human NEK6 protein produced in insect cells effectively phosphorylates histones H1 and H3, but not casein. Thus, these results suggest that, unlike other mammalian NIMA-related kinases, NEK6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. NEK6 transcripts are ubiquitously expressed with the highest expression found in the heart and skeletal muscle, and the hNek6 gene is localized to human chromosome 9q33-34. NEK6 has four isoforms with MW 35-40 kDa.

Protocols

Product Specific Protocols
WB protocol for NEK6 antibody 10378-1-AP	Download protocol
IF protocol for NEK6 antibody 10378-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Mol Biosci LncRNA FAM13A-AS1 Promotes Renal Carcinoma Tumorigenesis Through Sponging miR-141-3p to Upregulate NEK6 Expression. Authors - Xin Jun Wang View Article
human	WB	J Oncol MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells. Authors - Zhongshi Hong View Article
human	WB,IF	Int J Gen Med NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p Authors - Min Zhu View Article
human	WB	Cancers (Basel) Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus Authors - Lei Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEK6 Polyclonal antibody

NEK6 Polyclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 10378-1-AP

WB analysis of HEK-293 using 10378-1-AP

WB analysis of HT-1080 using 10378-1-AP

WB analysis of HeLa using 10378-1-AP

WB analysis using 10378-1-AP

WB analysis of HEK-293 using 10378-1-AP

WB analysis of HT-1080 using 10378-1-AP

IF Staining of HeLa using 10378-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LncRNA FAM13A-AS1 Promotes Renal Carcinoma Tumorigenesis Through Sponging miR-141-3p to Upregulate NEK6 Expression.

MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells.

NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus