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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEK9 Polyclonal antibody

NEK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11192-1-AP

Synonyms

NimA-related kinase 8, Never in mitosis A-related kinase 9, NERCC, Nek8, KIAA1995

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NEK9 Polyclonal antibody (11192-1-AP)

    WB analysis of HeLa using 11192-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NEK9 Polyclonal antibody (11192-1-AP)

    WB analysis using 11192-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using NEK9 Polyclonal antibody (11192-1-AP)

    WB analysis of human placenta using 11192-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NEK9 Polyclonal antibody (11192-1-AP)

    IP experiment of HeLa using 11192-1-AP

    IP result of anti-NEK9 (IP:11192-1-AP, 4ug; Detection:11192-1-AP 1:1000) with HeLa cells lysate 1880 ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NEK9 Polyclonal antibody (11192-1-AP)

    IHC staining of human lymphoma using 11192-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NEK9 Polyclonal antibody (11192-1-AP)

    IHC staining of human lymphoma using 11192-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NEK9 Polyclonal antibody (11192-1-AP)

    IF Staining of HepG2 using 11192-1-AP

    Immunofluorescent analysis of HepG2 cells, using NEK9 antibody 11192-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NEK9 Polyclonal antibody (11192-1-AP)

    IF Staining of HepG2 using 11192-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEK9 antibody (11192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using NEK9 Polyclonal antibody (11192-1-AP)

    FC experiment of HeLa using 11192-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NEK9 (11192-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11192-1-AP targets NEK9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK9 fusion protein Ag1678 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)- related kinase 9
Calculated molecular weight 107 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC009336
Gene symbol NEK9
Gene ID (NCBI) 91754
RRIDAB_2150800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as αβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704).

Protocols

Product Specific Protocols
WB protocol for NEK9 antibody 11192-1-APDownload protocol
IHC protocol for NEK9 antibody 11192-1-APDownload protocol
IF protocol for NEK9 antibody 11192-1-APDownload protocol
IP protocol for NEK9 antibody 11192-1-APDownload protocol
FC protocol for NEK9 antibody 11192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

JBMR Plus

Identification of Chloride Intracellular Channel Protein 3 as a Novel Gene Affecting Human Bone Formation.

Authors - Andrea M Brum
View Article
humanWB

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen
View Article