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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NELF Polyclonal antibody

NELF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12244-1-AP

Synonyms

NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NELF Polyclonal antibody (12244-1-AP)

    WB analysis of HeLa using 12244-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using NELF Polyclonal antibody (12244-1-AP)

    WB analysis of human colon using 12244-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NELF Polyclonal antibody (12244-1-AP)

    WB analysis of HeLa using 12244-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NELF Polyclonal antibody (12244-1-AP)

    WB analysis of mouse testis using 12244-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NELF Polyclonal antibody (12244-1-AP)

    WB analysis of human brain using 12244-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NELF Polyclonal antibody (12244-1-AP)

    WB analysis of mouse testis using 12244-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NELF Polyclonal antibody (12244-1-AP)

    IHC staining of human colon cancer using 12244-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12244-1-AP (NELF antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NELF Polyclonal antibody (12244-1-AP)

    IF Staining of HeLa using 12244-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12244-1-AP (NELF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human colon tissue, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12244-1-AP targets NELF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NELF fusion protein Ag2888 相同性解析による交差性が予測される生物種
Full Name nasal embryonic LHRH factor
Calculated molecular weight 507 aa, 56 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC016862
Gene symbol NELF
Gene ID (NCBI) 26012
RRIDAB_2150819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NSMF has some isoforms with MW 44 kDa and 55-60 kDa. This antibody recognizes all the isoforms.

Protocols

Product Specific Protocols
WB protocol for NELF antibody 12244-1-APDownload protocol
IHC protocol for NELF antibody 12244-1-APDownload protocol
IF protocol for NELF antibody 12244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols