Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83490-2-RR targets NELF in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | NELF fusion protein Ag34902 相同性解析による交差性が予測される生物種 |
| Full Name | nasal embryonic LHRH factor |
| Calculated molecular weight | 507 aa, 56 kDa |
| Observed molecular weight | 54-60 kDa |
| GenBank accession number | BC016862 |
| Gene Symbol | NELF |
| Gene ID (NCBI) | 26012 |
| RRID | AB_3671117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6X4W1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NELF plays a major role in promoter-proximal pausing, a widespread phenomenon during early transcription elongation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NELF antibody 83490-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |