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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neudesin/NENF Polyclonal antibody

Neudesin/NENF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15760-1-AP

Synonyms

Neudesin / NENF, Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neudesin/NENF Polyclonal antibody (15760-1-AP)

    WB analysis using 15760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15760-1-AP (Neudesin/NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15760-1-AP targets Neudesin/NENF in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neudesin/NENF fusion protein Ag8387 相同性解析による交差性が予測される生物種
Full Name neuron derived neurotrophic factor
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC008823
Gene symbol NENF
Gene ID (NCBI) 29937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).

Protocols

Product Specific Protocols
WB protocol for Neudesin/NENF antibody 15760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols