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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nestin Polyclonal antibody

Nestin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30760-1-AP

Synonyms

FLJ21841, Nbla00170, NES, nestin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (30760-1-AP)

    WB analysis using 30760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30760-1-AP (Nestin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSGC-7901 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30760-1-AP targets Nestin in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nestin fusion protein Ag29600 相同性解析による交差性が予測される生物種
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight240-280 kDa
GenBank accession numberNM_006617
Gene symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Protocols

Product Specific Protocols
WB protocol for Nestin antibody 30760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols