Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human colon tissue, human pancreas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
10150-2-AP targets GNAS in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GNAS fusion protein Ag0197 相同性解析による交差性が予測される生物種 |
| Full Name | GNAS complex locus |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC002722 |
| Gene Symbol | GNAS |
| Gene ID (NCBI) | 2778 |
| RRID | AB_2111668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95467 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAS antibody 10150-2-AP | Download protocol |
| IHC protocol for GNAS antibody 10150-2-AP | Download protocol |
| IF protocol for GNAS antibody 10150-2-AP | Download protocol |
| IP protocol for GNAS antibody 10150-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT
| ||
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
Sci Rep Sex-specific transgenerational effects on murine thyroid gland imposed by ancestral exposure to neonicotinoid thiacloprid | ||
Cell Mol Biol Lett GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation. | ||
Am J Physiol Renal Physiol Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.
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