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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using NET1 Polyclonal antibody (12740-1-AP)

    WB analysis of Y79 using 12740-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12740-1-AP (NET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NET1 Polyclonal antibody (12740-1-AP)

    IF Staining of A431 using 12740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NET1 antibody (12740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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NET1 Polyclonal antibody

NET1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12740-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Proto-oncogene p65 Net1, Proto oncogene p65 Net1, Neuroepithelial cell-transforming gene 1 protein, NET1A, ARHGEF8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12740-1-AP targets NET1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NET1 fusion protein Ag3636 相同性解析による交差性が予測される生物種
Full Name neuroepithelial cell transforming 1
Calculated molecular weight 596 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC010285
Gene Symbol NET1
Gene ID (NCBI) 10276
RRIDAB_2923558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NET1 antibody 12740-1-APDownload protocol
IHC protocol for NET1 antibody 12740-1-APDownload protocol
IF protocol for NET1 antibody 12740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Pathol

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation.

Authors - Yuting Zhang
View Article