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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using NEU1 Monoclonal antibody (67032-1-Ig)

    WB analysis of PC-12 using 67032-1-Ig (same clone as 67032-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NEU1 Monoclonal antibody (67032-1-Ig)

    WB analysis using 67032-1-Ig (same clone as 67032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat pancreas tissue using NEU1 Monoclonal antibody (67032-1-Ig)

    WB analysis of rat pancreas using 67032-1-Ig (same clone as 67032-1-PBS)

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using NEU1 Monoclonal antibody (67032-1-Ig)

    WB analysis of pig liver using 67032-1-Ig (same clone as 67032-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NEU1 Monoclonal antibody (67032-1-Ig)

    IHC staining of human pancreas cancer using 67032-1-Ig (same clone as 67032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67032-1-Ig (NEU1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NEU1 Monoclonal antibody (67032-1-Ig)

    IF Staining of U-251 using 67032-1-Ig (same clone as 67032-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 67032-1-Ig (NEU1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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NEU1 Monoclonal antibody, PBS Only

NEU1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67032-1-PBS
Clone No.1F6D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

N acetyl alpha neuraminidase 1, Lysosomal sialidase, G9 sialidase, EC:3.2.1.18, Acetylneuraminyl hydrolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67032-1-PBS targets NEU1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEU1 fusion protein Ag27945 相同性解析による交差性が予測される生物種
Full Name sialidase 1 (lysosomal sialidase)
Calculated molecular weight 45 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011900
Gene Symbol NEU1
Gene ID (NCBI) 4758
RRIDAB_2882347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99519
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).