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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEU2 Recombinant antibody

NEU2 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240685B3

Cat no : 83934-1-RR

Synonyms

SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NEU2 Recombinant antibody (83934-1-RR)

    WB analysis of mouse skeletal muscle using 83934-1-RR

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83934-1-RR (NEU2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NEU2 Recombinant antibody (83934-1-RR)

    IHC staining of mouse skeletal muscle using 83934-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NEU2 Recombinant antibody (83934-1-RR)

    IHC staining of mouse skeletal muscle using 83934-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83934-1-RR

    Affinity and Kinetic Characterization of 83934-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83934-1-RR against Human NEU2 were performed. The affinity constant is 2.76 nM.

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83934-1-RR is NEU2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEU2 fusion protein Ag17386 相同性解析による交差性が予測される生物種
Full Name sialidase 2 (cytosolic sialidase)
Calculated molecular weight 380 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC107053
Gene symbol NEU2
Gene ID (NCBI) 4759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)

Protocols

Product Specific Protocols
WB protocol for NEU2 antibody 83934-1-RRDownload protocol
IHC protocol for NEU2 antibody 83934-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols