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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEU2 Recombinant antibody, PBS Only

NEU2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240685C12

Cat no : 83934-2-PBS

Synonyms

SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using NEU2 Recombinant antibody (83934-2-RR)

    IF Staining of PC-12 using 83934-2-RR (same clone as 83934-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using NEU2 antibody (83934-2-RR, Clone: 240685C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83934-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using NEU2 Recombinant antibody (83934-2-RR)

    FC experiment of A549 using 83934-2-RR (same clone as 83934-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neu2 Recombinant Antibody (83934-2-RR, Clone:240685C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83934-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83934-2-PBS targets NEU2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEU2 fusion protein Ag17386 相同性解析による交差性が予測される生物種
Full Name sialidase 2 (cytosolic sialidase)
Calculated molecular weight 380 aa, 42 kDa
GenBank accession numberBC107053
Gene symbol NEU2
Gene ID (NCBI) 4759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)